Relationship between maternal emotional symptoms and emotional and behavioral problems of preschool offspring / 中国学校卫生

Objective@#To explore the relationship between maternal emotional symptoms and emotional and behavioral problems (EBPs) of preschool offspring, and to provide reference for prevention and intervention of emotional and behavior problems in preschool children.@*Methods@#A total of 4 100 preschool children aged 3-6 in Fuyang City were enrolled by stratified cluster sampling method in June 2021. The Strengths and Difficulties Questionnaire (SDQ) and Depression Anxiety and Stress Scale (DASS-21) were used to investigate preschool children s EBPs and maternal emotional symptoms, respectively.@*Results@#The detection rates of preschool children s emotional symptoms, conduct problems, hyperactivity, peer interaction problems, prosocial behavior and difficulty scores were 15.7%, 17.4%, 20.0%, 32.3%, 15.1% and 15.8%, respectively. The detection rates of mother s depression, anxiety and stress symptoms were 7.9%, 12.7% and 4.8%, respectively. Multivariate Logistic regression analysis showed that, compared with children of mothers with no depression, anxiety and stress symptoms, offspring of mothers reported depression, anxiety and stress symptoms had significantly higher risks of emotional symptoms, conduct problems, hyperactivity, peer problems and total difficulties ( OR=1.76-6.35, P <0.01). Maternal emotional symptoms had the most significant effect on children s emotional symptoms, and there was no significant correlation between maternal stress symptoms and children s prosocial behavior ( OR=1.40, P >0.05). There was no significant gender difference in the relationship between maternal emotional symptoms and children s emotional and behavioral problems ( ROR=0.73-1.07, P >0.05). For only children whose mothers reported stress symptoms showed a higher risk of emotional and behavior problems than non singleton children ( ROR=2.03, P <0.05).@*Conclusion@#Maternal emotional symptoms are related to preschool children s emotional and behavioral problems. Early identification and intervention of mother s negative emotional symptoms, are beneficial to the prevention of preschool children s emotional and behavioral problems.

Enzyme-linked immunosorbent assays for quantification of MMMAE-conjugated ADCs and total antibodies in cynomolgus monkey sera / 药物分析学报

Antibody-drug conjugates(ADCs)are commonly heterogeneous and require extensive assessment of exposure-efficacy and exposure-safety relationships in preclinical and clinical studies.In this study,we report the generation of a monoclonal antibody against monomethyl auristatin E(MMAE)and the development,validation,and application of sensitive and high-throughput enzyme-linked immunosor-bent assays(ELISA)to measure the concentrations of MMAE-conjugated ADCs and total antibodies(tAb,antibodies in ADC plus unconjugated antibodies)in cynomolgus monkey sera.These assays were suc-cessfully applied to in vitro plasma stability and pharmacokinetic(PK)studies of SMADC001,an MMAE-conjugated ADC against trophoblast cell surface antigen 2(TROP-2).The plasma stability of SMADC001 was better than that of similar ADCs coupled with PEG4-Val-Cit,Lys(m-dPEG24)-Cit,and Val-Cit linkers.The developed ELISA methods for the calibration standards of ADC and tAb revealed a correlation be-tween serum concentrations and the OD450 values,with R2 at 1.000,and the dynamic range was 0.3-35.0 ng/mL and 0.2-22.0 ng/mL,respectively;the intra-and inter-assay accuracy bias％ranged from-12.2％to-5.2％,precision ranged from-12.4％to-1.4％,and the relative standard deviation(RSD)was less than 6.6％and 8.7％,respectively.The total error was less than 20.4％.The development and validation steps of these two assays met the acceptance criteria for all addressed validation parameters,which suggested that these can be applied to quantify MMAE-conjugated ADCs,as well as in PK studies.Furthermore,these assays can be easily adopted for development of other similar immunoassays.

Evaluating the effectiveness of university agricultural extension test stations using Wuli-Shili-Renli methodology / Avaliação da eficácia de estações de teste de extensão agrícola universitária usando a metodologia Wuli-Shili-Renli

ABSTRACT: Wuli-Shili-Renli (WSR) methodology was used to assess the effects of systematic management on university agricultural extension test stations, in order to understand areas for further optimization. We developed a framework model and evaluation index system, of which, the weights of the evaluation index system were determined using AHP. Using an agricultural extension test station of Hebei Agricultural University as an example, we interviewed and surveyed in total of 225 university extension experts from nine test stations. Assessment showed that the test station system organization produces desirable societal benefits and improvements. In addition, the organizational structure and job allocation were found to act as high-impact factors in improving the optimal system function, with the university extension experts functional better in the incentive and guarantee mechanism than other factors. Our investigation validated that the university extension experts were critical in designing the incentives and evaluation programs.

RESUMO: A metodologia Wuli-Shili-Renli (WSR) foi usada para avaliar os efeitos do manejo sistemático nas estações de teste de extensão agrícola da universidade, a fim de entender as áreas para maior otimização. Desenvolvemos um modelo de estrutura e um sistema de índices de avaliação, dos quais os pesos do sistema de índices de avaliação foram determinados com o AHP. Usando uma estação de teste de extensão agrícola da Universidade Agrícola de Hebei como exemplo, entrevistamos um total de 225 especialistas em extensão universitária de nove estações de teste. A avaliação mostrou que a organização do sistema da estação de teste produz benefícios e melhorias sociais desejáveis. Além disso, verificou-se que a estrutura organizacional e a alocação de cargos atuam como fatores de alto impacto na melhoria da função ótima do sistema, com os especialistas em extensão universitária funcionando melhor no mecanismo de incentivo e garantia do que outros fatores. Nossa investigação validou que os especialistas em extensão universitária eram críticos no desenho dos incentivos e programas de avaliação.

Effect of TTTY10 regulating miR-490-3p on migration and invasion of cervical cancer cell via HMGB1 signaling pathway / 中国肿瘤生物治疗杂志

@# Objective：To investigate the effect of long-chain non-coding RNATTTY10 (lncRNATTTY10) on the migration and invasion of cervical cancer cells, and to explore its regulatory effect on miR-490-3p and HMGB1 (high mobility group box 1) signaling pathways. Methods: Fourteen paris of cervical cancer tissues and corresponding paracancerous tissues resected at the Department of Obstetrics and Gynecology,Affiliated Wuhan Central Hospital of Tongji Medical College fromAugust 2013 to December 2014 were collected for this study. The expression of TTTY10 in cervical cancer tissue and different cervical cancer cell lines were detected by qPCR. Plasmids encoding TTTY10-siRNA or empty plasmids were transfected into cervical cancer CasKicells, and the transfection efficiency was detected by qPCR. Transwell migration assay and Transwell invasion assay were used to detect the migration and invasion abilities of cervical cancer cells after TTTY10 silencing. qPCR was used to detect the expression of miR-490-3p and HMGB1 mRNA after TTTY10 silencing. Dual luciferase reporter assay validated the interaction between miR-490-3p and HMGB1. Western blotting was used to detect the expression of HMGB1 signaling pathway related proteins after TTTY10 silencing. Results: The expression of TTTY10 in cervical cancer tissues was significantly higher than that in paracancerous tissues (P<0.01), the expression of TTTY10 in cervical cancer cell lines was significantly higher than that in cervical epithelial cells (P<0.01). TTTY10-siRNAplasmids could efficiently transfectCasKicells to knockdown TTTY10 expression (P<0.01). Silencing of TTTY10 inhibited the migration and invasion of cervical cancer CasKi cells (P<0.05), promoted the expression of miR-490-3p (P<0.01) and inhibited the expression of HMGB1 mRNAin cervical cancer (P<0.05 or P<0.01). miR-490-3p could specifically bind to the 3'-UTR of HMGB1 mRNA(P<0.01). HMGB1 signaling pathway related proteins were down-regulated after TTTY10 silencing. Conclusion: TTTY10 can target regulate the expression of miR-490-3p and affect the migration and invasion ability of cervical cancer CasKi cells through the HMGB1 signaling pathway; TTTY10 can be used as a diagnostic marker and potential treatment target of cervical cancer.

The effects of different Er: YAG laser parameters combined with dole fluorine on dentinal tubules / 口腔疾病防治

Objective@# To explore the effects of Er: YAG lasers with different pulse energies and of Er: YAG lasers combined with Duraphat on dentine surface morphology and the dentinal tubule exposure rate, thereby providing a theoretical basis and parameters for clinically treating dentine hypersensitivity with Er: YAG lasers.@*Methods @# Dentinal tubule exposure models were divided into five groups, namely, group A (single Er: YAG laser exposure), group B (Duraphat+ Er: YAG laser), and group C (Er: YAG laser+Duraphat), which were exposed to 6 levels of pulse energy (80 mJ/P- 250 mJ/P), and experimental control group D (Duraphat group) and blank control group E. Scanning electron microscopy was used to observe dentine surface morphology and dentinal tubule closure in these groups, and Image-Pro Plus 6.0 software was used for image analysis to measure the exposure rates of the five groups of dentinal tubules.@*Results@#The dentinal tubule exposure rates for the A-D groups were significantly lower than that of the E group (P < 0.001); when the Er: YAG laser pulse energy was less than 250 mJ/P, the dentinal tubule exposure rates for the A-C groups were all lower than that of the D group, but when the pulse energy was higher than 250 mJ/P, the dentinal tubule exposure rates for the A-C groups were higher than that of the D group, showing significant differences (P < 0.001); under the same handling method, when the pulse energy was 80~200 mJ/P, the dentinal tubule exposure rate decreased as the pulse energy increased. If the pulse energy reached 250 mJ/P, the exposure rate of the dentinal tubules increased instead. There were apparently no significant differences between 80 mJ/P, 100 mJ/P, 160 mJ/P and 200 mJ/P in the A and C groups or between 80 mJ/P and 100 mJ/P in the B group (P > 0.05), while significant differences existed among the other groups (P< 0.05). Under the same pulse energy, the dentinal tubule exposure rates were A group > C group >B group; under different pulse energies, there existed significant differences between the A and B groups (P < 0.05); when the pulse energy was 120~250 mJ/P, there were significant differences between the B and C groups (P < 0.05), while there were no significant differences among other groups (P＜0.05).@*Conclusion@#Er: YAG lasers can close the dentinal tubules and play a cooperative role if combined with Duraphat; the effects of laser exposure are better if fluorine is applied beforehand; and dentinal tubule closure is positively correlated with the pulse energy of Er: YAG lasers within a certain energy scope, but when the pulse energy reaches 200 mJ/P, the dentine surface is excessively fused and injured.

Co‑existing ligneous conjunctivitis and IgG4‑related disease.

Herein, we elucidate that ligneous conjunctivitis (LC) was proved as an IgG4‑related disease (IgG4‑RD) by a series of pathologic studies from primary and recurrent episodes of an LC patient. LC was diagnosed based on clinical presentation and pathological appearance; furthermore, combined with serological examination and immunohistochemical study, the case also conformed to the diagnosis of IgG4‑RD. The IgG4‑RD, broadly discussed in recent times, is an idiopathic disease entity with tissue fibrosis possibly involving multiple organs. To the best of our knowledge, IgG4‑RD has never been reported with LC. By reporting the clinical course and literature review, we should pay attention to the association between these two diseases.